Name: Toshio Sakamoto,
Male
Date and Place of Birth: May 1, 1965, Tokyo
Address for
communication:
Department of Biology
Faculty of Science
Kanazawa University
Kakuma, Kanazawa 920-1192
E-mail: sakamot@kenroku.kanazawa-u.ac.jp
TEL: +81(76) 264-6227; +81(76)-264-6308
FAX: +81(76) 264-6215
Education:
1985-1989: B.Sc. Dept. of Biophys. and Biochem.
Fac. of Sci., University of Tokyo
1989-1991: M.Sc. Dept. of Biophys. and Biochem.
Fac. of Sci., University of Tokyo
1991-1994: Dr.Sc. Dept. of Molecular Biomechanics,
Graduate University of Advanced Studies
Title of thesis:
Fatty acid desaturases of cyanobacteria and the modification of membrane lipid unsaturation.
(Supervisor: Dr. Norio Murata)
History:
1994-1995: JSPS Postdoctoral fellow (Dr. M. Ohmori)
Dept. of Biol., College of Arts and Sci., University of Tokyo
1995-2000: Postdoctoral fellow (Dr. D.A. Bryant)
Dept. of Biochem. and Molec. Biol., Pennsylvania State University
(Recipient of fellowship of Yamada Foundation, 1995)
2000-2003: Assistant professor, Dept. of Biol., Kanazawa University
2004-present: Associate professor, Graduate School of Natural Science and Technology, Kanazawa University
Publications:
Original papers:
1) Sakamoto, T., Murata, N. and Ohmori, M.
(1991) The concentration of cyclic AMP and adenylate cyclase activity
in cyanobacteria. Plant Cell Physiol., 32:581-584.
2) Wada, H., Gombos, Z, Sakamoto, T. and Murata, N. (1992) Genetic
manipulation of the extent of desaturation of fatty acids in membrane
lipids in the cyanobacterium Synechocystis PCC6803.
Plant Cell Physiol. 33:535-540.
3) Malakhov, M. P., Wada, H., Los, D. A, Sakamoto, T. and Murata, N.
(1993) Structure of a cyanobacterial gene encoding the 50S ribosomal
protein L9. Plant Mol. Biol. 21:913-918.
4) Sakamoto, T.,
Wada, H., Nishida, I., Ohmori, M. and Murata, N. (1994)
Identification of conserved domains in the Delta-12 desaturases of
cyanobacteria. Plant Mol. Biol. 24:643-650.
5) Sakamoto, T., Los, D.
A., Higashi, S., Wada, H., Nishida, I., Ohmori, M. and Murata, N.
(1994) Cloning of omega-3 desaturase from cyanobacteria and its use
in altering the degree of membrane-lipid unsaturation. Plant Mol.
Biol. 26:249-263.
6) Sakamoto, T., Wada, H.,
Nishida, I., Ohmori, M. and Murata, N. (1994) Delta-9 acyl-lipid
desaturases of cyanobacteria: Molecular cloning and substrate
specificities in terms of fatty acids, sn-positions, and polar head
groups. J. Biol. Chem. 269:25576-25580.
7) Sakamoto, T. and Murata, N. (1995) Sequence announcement of EMBL
accession #: D13777 Plant Mol. Biol. 29:187.
8) Yashiro, K., Sakamoto, T. and Ohmori, M. (1996) Molecular
characterization of an adenylate cyclase gene of the cyanobacteriumSpirulina platensis. Plant Mol. Biol. 31:175-181.
9) Sakamoto, T. and
Bryant, D. A. (1997) Temperature-regulated mRNA accumulation and
stabilization for fatty acid desaturase genes in the cyanobacterium
Synechococcus sp. strain PCC 7002. Molec. Microbiol.
23:1281-1292.
10) Sakamoto, T., Higashi,
S., Wada, H., Murata, N. and Bryant, D.A. (1997) Low-temperature-
induced desaturation of fatty acids and expression of desaturase
genes in the cyanobacterium Synechococcus sp. PCC
7002. FEMS Microbiol. Lett. 152:313-320.
11) Gombos, Z., Kanervo, E., Tsvetkova, N., Sakamoto, T., Aro, E-M.
and Murata, N. (1997) Genetic enhancement of the ability to tolerate
photoinhibition by introduction of unsaturated bonds into membrane
glycerolipids. Plant Physiol. 115:551-559.
12) Kasahara, M., Yashiro, K., Sakamoto, T. and Ohmori, M. (1997) TheSpirulina platensis adenylate cyclase gene, cyaC, encodes a novel
signal transduction protein. Plant Cell Physiol. 38:828-836.
13) Sakamoto, T.
and Bryant D.A. (1998) Growth at low temperature causes nitrogen
limitation in the cyanobacterium Synechococcus sp. PCC
7002. Arch. Microbiol. 169:10-19.
14) Sakamoto, T., Shen,
G., Higashi, S, Murata, N. and Bryant, D.A. (1998) Alteration of
low-temperature susceptibility of the cyanobacterium Synechococcus sp. PCC 7002
by genetic manipulation of membrane lipid unsaturation. Arch.
Microbiol. 169:20-28.
15) Sakamoto, T. Delgaizo,
V.B. and Bryant, D.A. (1998) Growth on urea can trigger death and
peroxidation of the cyanobacterium Synechococcus sp. strain
PCC 7002. Appl. Env. Microbiol. 64:2361-2366.
16) Sakamoto, T. and
Bryant, D.A. (1999) Nitrate transport and not photoinhibition limits
growth of the freshwater cyanobacterium Synechococcus species PCC
6301 at low temperature. Plant Physyiol. 119:785-794.
18) Sakamoto, T. and Bryant, D.A. (2001) Requirement of nickel as an essential micronutrient for the utilization of urea in the marine cyanobacterium Synechococcus sp. PCC 7002. Microbes and Environments 16:177-184.
19) Nakajima, M., Sakamoto, T. and Wada, K. (2002) The complete purification and characterization of three forms of ferredoxin-NADP+ oxidoreductase from a thermophilic cyanobacterium Synechococcus elongatus. Plant Cell Physiol. 43:484-493.
20) Sakamoto, T. and Bryant, D.A. (2002) Synergistic effect of high-light and low temperature on cell growth of the delta-12 fatty acid desaturase mutant in Synechococcus sp. PCC 7002. Photosynthesis Research 72:231-242.
21) Tamaru, Y., Takani, Y., Yoshida, T. and Sakamoto, T. (2005) Crucial role of extracellular polysaccharides in desiccation and freezing tolerance in the terrestrial cyanobacterium Nostoc commune. Applied and Environmental Microbiol. 71:7327-7333.
22) Chintalapati, S., Prakash, J. S. S., Gupta, P., Ohtani, S., Suzuki, I., Sakamoto, T., Murata, N. and Shivaji, S. (2006) A novel delta-9 acyl-lipid desaturase, DesC2, from cyanobacteria acts on fatty acids esterified to the sn-2 position of glycerolipids. Biochemical J. 368:207-214.
24) Inoue-Sakamoto, K., Gruber, T.M., Christensen, S.K., Arima, H., Sakamoto, T. and Bryant, D.A. (2007) Group 3 sigma factors in the marine cyanobacterium Synechococcus sp. strain PCC 7002 are required for growth at low temperature. J. Gen. Appl. Microbiol. 53:89-104.
25) Morsy, F.M., Nakajima, M., Yoshida, T., Fujiwara, T., Sakamoto, T. and Wada, K. (2008) Subcellular localization of ferredoxin-NADP+ oxidoreductase in phycobilisome retaining oxygenic photosynthetic organisms. Photosynth. Res. 95:73-85.
Proceedings:
1) Sakamoto, T., Stirewalt, V.L. and Bryant D.A. (1996) Two acyl-lipid delta-9 desaturase genes of the cyanobacterium, Synechococcus sp. strain PCC7002. In: Physiology, Biochemistry and Molecular Biology of Plant Lipids (Williams, J.P., Mobashsher, U. and Lem, N.W. eds.) pp.380-382. Kluwer Academic Publishers, Dordrecht.
Review artilces:
1) Sakamoto, T. and Murata, N. (2002) Regulation of the desaturation of fatty acids and its role in tolerance to cold and salt stress. Current Opinion in Microbiology 5:206-210.